Question: How do you dispose of laboratory waste and culture?

How do you dispose of cell culture waste?

Cell Culture Waste Disposal

  1. Tissue culture waste (culture medium) – inactivate for at least 2 hours in a solution of hypochlorite (10,000ppm) prior to disposal to drain with an excess of water.
  2. Contaminated pipettes should be placed in hypochlorite solution (2500ppm) overnight before disposal by incineration.

How do you dispose biological wastes in the laboratory?

Liquid biological waste should be collected in containers for autoclaving or chemical disinfection. Autoclaved or chemically disinfected liquid wastes can be disposed via the laboratory sink. Do not pour melted agarose down the drain.

How do you dispose of culture media?


Any apparatus used and contaminated must be safely disinfected or sterilised. It is suggested that decontamination should be carried out by autoclaving at 121°C for 60 minutes. After autoclaving, media may be disposed of through normal waste disposal or incineration procedures.

What is the proper way to dispose of infectious waste?

Proper sanitation or disposal

  1. Minimise contact, i.e. handle materials as little as possible and transfer them via routes that minimise exposure to others.
  2. Discard materials into a suitable container or bag. …
  3. Use bags that are marked or coloured for infectious waste.
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How do you dispose of cultures?

Throw the culture away in the garbage container marked with a biohazard sign. c. Allow the agar to dehydrate in the incubator and then discard in any garbage. d.

What is infectious waste and how is is disposed?

Infectious wastes must either be incinerated or treated prior to disposal. o BIOLOGICAL WASTE includes blood and blood products, excretions, exudates, secretions, suctionings and other. body fluids that cannot be directly discarded into the municipal sewer system, but EXCLUDES articles contaminated.

How do you handle laboratory waste?

Laboratory Waste Disposal Procedure Summary

  1. Do not place any liquids in the municipal waste.
  2. Do not dispose of chemical waste, including stock containers with unused product, in the municipal waste.
  3. Empty or rinsed containers must be free of any hazardous residue and be marked “empty.”

What are laboratory wastes?

Laboratory waste is waste that is generated from laboratories in industry and in educational centres such as secondary schools and universities. This waste can be broken down into a number of categories: Hazardous; Clinical; Biological; Electrical; Laboratory.

Why proper disposal of used media of laboratory is important?

Radioactive materials: It is essential that you properly dispose of any radioactive materials, such as those used in research and to diagnose and treat certain kinds of Cancers. These materials should be stored in a closed container that is kept isolated and at a distance from the workspace.

What precautions can be used when handling waste?

Wear heavy-duty gloves and protective clothing when handling waste bags and containers. Avoid manual handling of waste where possible. Do not overfill bags or containers. Seal bags by tying off or stapling when 3/4 full.

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How do you dispose of clinical waste in a care setting?

If the healthcare waste does contain chemicals, even small traces, you should put it in yellow waste containers. It will then be disposed of by incineration in a suitably permitted or licensed facility. If the waste doesn’t contain any chemicals or pharmaceuticals, you can place it in orange bags or lidded receptacles.